cDNA autoradiograph images Best answer on the web

January 9, 2009 on 7:27 am | In mybachcars.com |
cDNA autoradiograph images Best answer on the web
  • I would consider it a real favor if you could look around for a database for cDNA autoradiograph images.
    The specific ones I'm looking for are Clontech Atlas arrays, especially Mouse cDNA Expression Arrays. Check out
    http://home.fujifilm.com/products/science/bas1800_2/images.html

    for an example. Anything that looks similar will be considered, but please just ask me for clarification before you answer if you are not sure about it.
    I'm hoping they will be in .tiff format.

    Thanks all!

    -rook


  • I think I may have found the types of website you seek and provide snippets of text from each one below. If thems seems OK, let me know and I'll post them.
    1)set of 18,816 full-length enriched mouse cDNA arrays Proceedins of ... Y., and Okazaki, Y. READ: RIKEN Expression Array Database Nucleic Acids


    2)stores raw and normalized data from microarray experiments, as well as their corresponding image files
    3)Mouse
    Note: For a searchable database of the mouse gene list, go to


  • If I am reading this correctly (and I'm not sure that I am!), this site:
    http://www.integriderm.com/download.shtml

    includes a document called "Methods for Gene Expression Profiling with Nylon Filter Microarrays", along with two downloadable file -- one with gene expression data, and the other (and this is where I'm not 100% sure) that converts the data into the type of images you are seeking:
    Pathways input Filter Library for DermArray -- PWI File

    Gene List -- To be imported into a spreadsheet program such as Excel to analyze experimental data
    The "Pathways" file needs certain software that you may or may not have access to.
    Please let me and the other researchers know if this type of information is useful, and how close (or distant) it is from what you actually need.
    Thanks.


  • At Mouse Genome Informatics, there are 146 results for a search on cDNA expression. I tried a couple of the links and they require a subscription to read the article. Probably don't contain pics but you never know...
    http://www.informatics.jax.org/searches/reference_report.cgi?text%3Atitle=&op%3Achromosome=in&op%3Apgs=begins&_summary=on&op%3Ajournal=begins&op%3A_primary=begins&list%3Achromosome=&text%3AreferenceID=&text%3Apgs=&int%3Ayear=&_GETAsPOST=true&text%3Ajournal=&text%3Aauthors=&op%3Aauthors=contains&op%3Atitle=contains&op%3Avol=%3D&text%3Aabstract_search=*cDNA+Expression*&_sort=year+desc%2C+_primary&te t%3A_primary=&op%3Ayear=%3D&text%3Avol=


  • Probably not exactly what you're after, but perhaps these are close enough. Have a look, and let me know (there are likely many more to be had like these --I took a quick look around):
    http://www.clontech.com/archive/JAN98UPD/Atlasarticle.shtml

    http://www.clontech.com/archive/JAN02UPD/pdf/AppNote.pdf [figure 3]

    http://www.clontech.com/archive/JUL01UPD/pdf/AtlasHuPlastic.pdf

    http://images.cellpress.com/supmat/cub/bb11_15Belham_1155.pdf


  • Close!....I just need a number of high quality mouse (or human) cDna expression array images. I hope this database will also name the "radioisotope" that was used to burn the image (i.e., phosphor 32 or 33).
    Check out the picture: "Membrane (8x12cm): Atlas™ cDNA Mouse Expression Array manufactured by CLONTECH Laboratories, Inc. Acquired by Fujifilm" located at the URL:
    http://home.fujifilm.com/products/science/bas1800_2/images.html

    Thanks for the try, though!


  • Close, rajeevsmind-ga, but I am looking for a radioisotope labelled (onto a nylon filter (aka membrane)) cDna expression array, the kind in the picture that I referenced on the ClonTech page. You were helpful, but your image databases had non-nylon filter pictures (in other works, they weren't using radioisotope labelling). Even if the images weren't created with CloneTech Atlas imagers, as long as they were radiolabelled cDna "spot" images that were captured on a nylon substrate (filter), the kind in the picture that I referenced on the ClonTech page.
    This database has to be out there somewhere. I just called the Stanford database guys because of rajeevsmind-ga's suggestion, and they said a French CNRS institute has the database, but I couldn't find it on the CNRS Web site. It's in French, so you might have to use the google language tools or contact CNRS directly if you know French.
    Thank you, rajeevsmind-ga, for trying!


  • Hi - most expression data is much more simply represented in a database by storing relative expression numbers tabularly, rather than graphically in enough resolution for any use (1-2 megs per blot, typical minimum). There are many public and private mouse gene expression databases, many with the genes that can be measured with Clontech arrays. Are you looking for pictures of the use of the Clontech arrays, or are you hoping to find a database of Clontech images that you can attempt to mine for expression data? If the latter is the case, I doubt that you will find an online database of the data stored as image files due to the large resolution needed for proper image analysis. There are however, many databases with tabular expression data.


  • Thanks pafalafa, but I am looking for a database of cDNA autoradiograph images (very similar to the examples on the CloneTech page), a database preferably of Mouse cDNA Expression Arrays. I do appreciate your attempt to assist!